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recombinant human rank tnfrsf11a fc chimera  (R&D Systems)


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    Structured Review

    R&D Systems recombinant human rank tnfrsf11a fc chimera
    Recombinant Human Rank Tnfrsf11a Fc Chimera, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/683-RK/Recombinant+Human+RANK%2FTNFRSF11A+Fc+Chimera+Protein%2C+CF/pm41862435-182-0-9
    Average 94 stars, based on 4 article reviews
    recombinant human rank tnfrsf11a fc chimera - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Recombinant:

    Article Title: TSP-1 interaction with RANK and OPG: implications for bone remodeling and osteolytic bone metastasis.
    Article Snippet: .. Recombinant human RANK/TNFRSF11A Fc Chimera and OPG/TNFRSF11B were from R&D System (Milan, Italy). .. Recombinant human RANK/TNFRSF11A Fc Chimera and OPG/TNFRSF11B were from R&D System (Milan, Italy).

    Article Title: TSP-1 interaction with RANK and OPG: implications for bone remodeling and osteolytic bone metastasis
    Article Snippet: .. Recombinant human RANK/TNFRSF11A Fc Chimera and OPG/TNFRSF11B were from R&D Systems (Milan, Italy). .. Murine recombinant murine RANK-L was from PeproTech (Thermo Fisher Rodano, Italy).

    Article Title: A Modified Glycosaminoglycan, GM-0111, Inhibits Molecular Signaling Involved in Periodontitis.
    Article Snippet: After 1 hr of incubation at room temperature, the plate was washed with PBS-T and 100 μL of horseradish peroxidase (HRP)-conjugated anti-goat IgG (ThermoScientific, cat# 31402) was added. .. Polyvinyl 96-well plates were coated with 500 ng/well of recombinant human RANK (cat# 683-RK, R&D systems). .. The enzymatic reaction was stopped with 1 NHCl, and the absorbance at 450 nm was measured using a microplate reader (SpectraMax, Molecular Devices, CA).

    Article Title: CCN2 enhances RANKL-induced osteoclast differentiation via direct binding to RANK and OPG.
    Article Snippet: Abbreviations: CTGF, connective tissue growth factor; assay; GST, glutathione transferase;M-CSF,macrophage co nuclear factor-kappa B; OPG, osteoprotegerin; RANK, rece receptor activator of NF-κB ligand; RANKL-GST, GST fusio resonance; TACE, TNF-α-converting enzyme; TRAP, tartra phatase; TRANCE, TNF-related activation-induced cytokine vated T-cells, cytoplasmic 1.. ⁎ Corresponding author at: Advanced Research Cen Sciences, Okayama University Dental School, Okayama Kita-ku, Okayama 700-8525, Japan.. Fax: +81 86 235 684 E-mail address: takigawa@md.okayama-u.ac.jp (M. Ta

    Article Title: A Modified Glycosaminoglycan, GM-0111, Inhibits Molecular Signaling Involved in Periodontitis
    Article Snippet: .. Polyvinyl 96-well plates were coated with 500 ng/well of recombinant human RANK (cat# 683-RK, R&D systems). ..

    FACS:

    Article Title: Activated Human T Cells Express Alternative mRNA Transcripts Encoding a Secreted Form of RANKL
    Article Snippet: For intracellular flow cytometry, Brefeldin A (Sigma-Aldrich) was added 4 hrs before harvest to some Jurkat T cells stimulated with PMA/ionomycin as well as control cells. .. Cells were resuspended in FACS buffer and then incubated with either IgG 1 -Fc (110-HG) or RANK-Fc (Cat # 683-RK) chimeras (5 μg/ml, R &D Systems) for 45 min on ice, washed twice for 30 min on ice in FACS buffer containing, phyco-erythin conjugated goat anti-human IgG (Fcγ fragment specific, (eBioscience, Cat # 12-4998, San Diego, CA, USA) for 30 min on ice in FACS buffer. .. Cells were resuspended in FACS buffer and then incubated with either IgG 1 -Fc (110-HG) or RANK-Fc (Cat # 683-RK) chimeras (5 μg/ml, R &D Systems) for 45 min on ice, washed twice for 30 min on ice in FACS buffer containing, phyco-erythin conjugated goat anti-human IgG (Fcγ fragment specific, (eBioscience, Cat # 12-4998, San Diego, CA, USA) for 30 min on ice in FACS buffer.

    Incubation:

    Article Title: Activated Human T Cells Express Alternative mRNA Transcripts Encoding a Secreted Form of RANKL
    Article Snippet: For intracellular flow cytometry, Brefeldin A (Sigma-Aldrich) was added 4 hrs before harvest to some Jurkat T cells stimulated with PMA/ionomycin as well as control cells. .. Cells were resuspended in FACS buffer and then incubated with either IgG 1 -Fc (110-HG) or RANK-Fc (Cat # 683-RK) chimeras (5 μg/ml, R &D Systems) for 45 min on ice, washed twice for 30 min on ice in FACS buffer containing, phyco-erythin conjugated goat anti-human IgG (Fcγ fragment specific, (eBioscience, Cat # 12-4998, San Diego, CA, USA) for 30 min on ice in FACS buffer. .. Cells were resuspended in FACS buffer and then incubated with either IgG 1 -Fc (110-HG) or RANK-Fc (Cat # 683-RK) chimeras (5 μg/ml, R &D Systems) for 45 min on ice, washed twice for 30 min on ice in FACS buffer containing, phyco-erythin conjugated goat anti-human IgG (Fcγ fragment specific, (eBioscience, Cat # 12-4998, San Diego, CA, USA) for 30 min on ice in FACS buffer.

    Article Title: Soluble RANKL contributes to osteoclast formation in adult mice but not ovariectomy-induced bone loss
    Article Snippet: Serum was incubated with Protein A-Sepharose 4B (101090, Thermo Fisher Scientific) for 5 h at 4 °C to pre-absorb immunoglobulins. .. RANK-Fc (683-RK-100, R&D systems) or IgG (sc-2025, Santa Cruz) was then coupled to Protein A-Sepharose 4B and incubated with the pre-absorbed serum samples at 4 °C overnight. ..

    96 Well Plates:

    Article Title: A Modified Glycosaminoglycan, GM-0111, Inhibits Molecular Signaling Involved in Periodontitis
    Article Snippet: .. Polyvinyl 96-well plates were coated with 500 ng/well of recombinant human RANK (cat# 683-RK, R&D systems). ..

    other:

    Article Title: RANK pathway inhibition sensitizes triple-negative breast cancer to CDK4/6 inhibitors and enhances immune response
    Article Snippet: All the cells were maintained at 37°C with 5% CO2, used at a low passage number, 415 and tested for Mycoplasma contamination via qPCR (Eurofins Genomics).



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